Skip to main content
. 2010 Apr 23;107(20):9035–9042. doi: 10.1073/pnas.0914013107

Fig. 4.

Fig. 4.

Combined bax/bak ablation protects mitochondrial-, but not reticular-, Nix-expressing cells from death. (A) Confocal imaging of cytochrome c (green) and mitochondrial (red) colocalization in bax-bak double null MEFs expressing Nix and Nix mutants. (Top) Vehicle. (Bottom) Cyclosporin A-treated. (B) Confocal imaging of annexin V staining (green) and TMRE (red) in identically treated cells. (C) Group data for cell death (white bars), annexin V labeling (black bars), and TUNEL positivity (gray bars) in bax-bak double null MEFs expressing Nix mutants (n = 6 per group). (Scale bars: 50 μm.) *, P < 0.05 vs. identically treated β-gal controls.