Fig. 2.

Regional distribution of T regulatory cells in relation to first syngenic mating. Single-cell suspensions from indicated lymphoid organs prepared from virgin (n = 3) or timed first pregnancies from C57BL/6 syngenic mating (dpc 6.5, n = 4; dpc 12.5, n = 6; dpc 18.5, n = 6), or retired from syngenic breeding (n = 4). FACS performed with gating on CD4⁺ cells to determine the fraction of FoxP3⁺ cells. Statistical differences determined by one-way ANOVA with Bonferroni–Dunn posttest (N.S., nonsignificant).