In vivo effect of denileukin diftitox on Treg populations in regional lymphoid organs. Shown is FACS analysis of indicated single-cell suspensions immediately ex vivo stained for CD4 and FoxP3 from timed first pregnancy of C57BL/6 × C57BL/6 at dpc 18.5. Saline, n = 6; denileukin diftitox, n = 4. Statistical difference was determined by two-tailed, unpaired Student's t test (N.S., nonsignificant; *, P < 0.05).