FIG. 2.

Linear regression analysis of p53 expression in melanoma cell lines and IFN-γ production by cognate T cells. Twelve of the 13 HLA-A2⁺ melanoma cell lines analyzed in Fig. 1 and Table 1 were included with the 2 HLA-A2⁺p53⁺ tumor control lines (SaOS2/143 and MDA-231) in a linear regression analysis investigating the relationship between the specific MFI of p53 and IFN-γ production by cognate effectors from patient 1. Tumor cell line 624 was excluded from this analysis because of the close proximity of the amino acid change to the p53_264–272 epitope. This analysis is representative of results obtained with IFN-γ values by p53:264 TCR-transduced T cells from patient 2 (R² = 0.0013, p > 0.1), and an analysis performed by excluding the outliers MDA-231 and SaOS2/143.