Fig. 2.

Inhibition of IRBP1177–1191-induced EAU with RTL220 in Lewis rats. Time course of EAU in RTL-treated rats with 5 doses of 300 μg of RTL220, RTL101 or RTL203 subcutaneously starting on the day of IRBP immunization (a) or at the onset of clinical signs (b). The data present an average of 10 eyes for 5 rats and SD from a representative experiment. The severity of inflammation was determined as described in the methods. The significance between the controls and treatment groups was determined by 1-way ANOVA, overall p = 0.0004. c Overall severity of EAU. Bars indicate CDIs calculated for each group (n = 5) in this experiment. d Level of cytokines in individual eyes from unimmunized rats (control), immunized with IRBP peptide (untreated) and not treated, and RTL220-treated rats (RTL220) from rats treated at the onset of clinical signs (n = 4 eyes). Beadlyte multiplex cytokine detection kit (Millipore) was used to measure cytokines as described in the methods. The significance between the control and treatment group was determined using Student's t test (∗ p <0.05) and is denoted by an asterisk. e Summary of histology scores for each group. Bars represent average histology scores for anterior and posterior segments of all eyes (n = 10) in experimental groups. f Representative histology of the retina from different treatment groups as shown in a and b. Arrows indicate inflammatory cells and retinal waves.