Fig. 4.

Levels of systemic and local chemokines in R-EAU in RTL220-treated rats in comparison to the untreated rats. Eyes (d–f) and spleens (a–c) were collected at the end of the experiment on day 39 after T cell transfer. Untreated (first bar) rats, rats treated with RTL220 starting at the first onset (second bar) or starting at the second onset (third bar). Chemokines were extracted from homogenized eyes or from 48 h IRBP-stimulated T cell supernatants for CCL2, CCL3 and CCL5 measurements and analyzed with a multiplex ELISA. A Beadlyte multiplex cytokine detection kit (Millipore) was used to measure cytokines as described in the methods and in figures 2 and 3. The significance between the control and treatment group (n = 4) was determined using Student's t test (∗ p <0.05 denoted by an asterisk).