Fig. 5.
The metabolic activity of recombinant CYP6BQ9 enzyme. (A) Rate of dealkylation reaction of recombinant CYP6BQ9 with the fluorescent substrate BOMR (Vivid; Invitrogen). The Km value was determined as 4.8 ± 0.9 μM. (B) In vitro activity assays with recombinant CYP6BQ9. Microsomes from Sf9 cells expressing QTC279 CYP6BQ9 and D. melanogaster CPR were incubated with deltamethrin for 3 h followed by mass spectrometric analyses. The empty baculovirus vector containing cells were used as a control. The peak area of the major metabolite (+16) was quantified. The data shown are mean + SEM (n = 3). (C) Mass spectrum of 4-hydroxy deltamethrin, the only metabolite detected in in vitro experiments incubating deltamethrin and CYP6BQ9 reconstituted protein complex.