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. 2010 Apr 26;107(19):8812–8817. doi: 10.1073/pnas.1001866107

Fig. 5.

Fig. 5.

Interaction between FliT and the FlhDC complex. (A) Swarming motility of SJW1103 (WT) transformed with pGEX-6p-1–based plasmids: GST, pGEX-6p-1–expressing GST; GST-FliT, N-terminally GST-tagged FliT(2-122); GST-FliT94, and N-terminally GST-tagged FliT(2-94). The plate was incubated at 30 °C for 6 h. (B) Pull-down assay of FlhC and FlhD by GST affinity chromatography. The soluble fractions from WT cells overproducing GST, GST-FliT, or GST-FliT94 were loaded onto a GST column. The eluted factions were analyzed by CBB staining for GST, GST-FliT, and GST-FliT94 (Top) and by immunoblotting for FlhC and FlhD with polyclonal anti-FlhC (Middle) and FlhD antibodies (Bottom), respectively. (C) Effect of FliD on the interaction between GST-FliT94 and the FlhDC complex.