Skip to main content
. 2010 Apr 21;107(19):8830–8835. doi: 10.1073/pnas.0910644107

Fig. 3.

Fig. 3.

Reduced spine density in eB3−/− mice. (A) Cortical neuron dendrites in slice culture from P4-6 wt or eB3−/− mice transfected with GFP. Arrows indicate spines; arrowheads indicate filopodia-like protrusions. (B–E) Quantification of protrusion density for (B) all protrusions, (C) spines, (D) filopodia-like protrusions, and (E) protrusions classified as “other” for wt (n = 16) or eB3−/− (n = 20) neurons. (F) (Top) Images of layer 5 cortical cryosections from P6 wt or eB3−/− mice immunostained with anti-VGlut1 (green) and anti-SynGAP (red) antibodies. (Scale bar, 10 μm.) (Bottom) Binary mask illustrating colocalized VGlut1 and SynGAP puncta in above images. (G) Quantification of colocalized VGlut1 and SynGAP puncta density in wt (n = 59 images) and eB3−/− (n = 60 images) sections. (H) DIV-4–6 cortical neuron dendrites in slice culture from P4-6 wt or eB3−/− mice transfected with tdTomato (red) and PSD-95-GFP (green) at DIV 2. Arrows indicate PSD-95-GFP puncta in dendritic protrusions. Arrowheads indicate PSD-95-GFP puncta on the dendritic shaft. (I and J) Quantification of puncta density for (I) PSD-95-GFP puncta found in protrusions and (J) total PSD-95-GFP puncta for wt (n = 12) or eB3−/− (n = 39) neurons. (Scale bars, 3 μm.) Error bars indicate SEM. *P < 0.002.