Skip to main content
. 2010 Apr 21;107(19):8830–8835. doi: 10.1073/pnas.0910644107

Fig. 5.

Fig. 5.

EphB2 is a presynaptic ligand for eB3. (A) Quantification of induction of VGlut1 puncta area in axons under HEK293T cells transfected with vector control (n = 78), HAeB1 (n = 70), HAeB2 (n = 63), HAeB3 (n = 68), or eA1 (n = 23). (B) DIV-10 axons from cortical neurons transfected with synaptophysin-GFP (green) and indicated shRNA constructs cocultured with HEK293T cells transfected with RFP or HAeB3 (red) for 16–18 h. Arrowheads indicate synaptophysin-GFP puncta colocalized with HEK293T cells. (Scale bar, 3 μm.) (C) Quantification of fold increase in synaptophysin–GFP density in axon segments underneath HEK293T cells compared with adjacent axon segments for neurons transfected with indicated shRNA constructs (cocultured with RFP-expressing HEK293T cells, HAeB3-expressing HEK293T cells): vector control (RFP: n = 111, HAeB3: n = 86), EphB2 shRNA#1 (RFP: n = 81, HAeB3: n = 85), EphB2 shRNA#2 (RFP: n = 41, HAeB3: n = 45), EphB2 shRNA#2 + EphB2R (RFP: n = 55, HAeB3: n = 58). Error bars indicate SEM. *P < 0.006.