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. 2010 Apr 26;107(19):8788–8793. doi: 10.1073/pnas.1003428107

Fig. 2.

Fig. 2.

Glutamine catabolism by mitochondria is required for oncogenic Kras-induced anchorage-independent growth. (A) Analysis of soft agar colonies of HCT116 cells in media containing 4 mM glutamine with 20 mM glucose or 20 mM galactose in the presence of 2 mM aminooxyacetic acid (AOA) and 7 mM dimethyl α-ketoglutarate (DMK). Mean ± SE (n = 9). **P < 0.01. (B) Oxygen consumption rate (OCR) was measured in HCT116 cells incubated in basal media and subsequently exposed to glutamine, AOA, and DMK. (C) Analysis of soft agar colonies of HCT116 cells expressing control and alanine amiotransferase 2 (ALT2) shRNA #1 and #2 ± DMK or (D) control and glutaminase 1 (GLS1) shRNA #1 and #2 ± DMK. Bars represent the mean ± SE (n = 16 in C and n = 8 in D). **P < 0.01.