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EPR spectra of deoxy I107E FeBMb (0.5 mM) with no metal bound in the presence of NO after 5 min (top line), with 2 eq Fe2+ (A), Cu+ (B), or Zn2+ (C) incubated with excess NO (∼200 eq) for 1, 5, and 15 min. Spectra were collected in 50 mM Bis-Tris pH 7.0 at 30 K, 0.2 mW power, and 9.05 GHz.
