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TFP induces S100A4 oligomerization. (A) Plot of fraction S100A4 pentamer versus TFP concentration. Sedimentation equilibrium data were collected at 25 °C at a concentration of 80 μM S100A4 subunit. (B) Chemical cross-linking of Ca2+-S100A4. Coomassie-stained SDS-PAGE of GST control, S100A4 alone, S100A4 + DMSO, and S100A4 + TFP. Monomeric and dimeric S100A4 have apparent molecular weights of approximately 11.5 kDa and 23 kDa, respectively.
