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SK-N-SH neuroblastoma cells coincubated with 5 μM Cu2(FL2E), 100 nM 17-β-estradiol, 0.2 μM Mitotracker Red, and 4.5 μM Hoechst 33258 for 30 min. (A) DIC image; (B) emission from the blue channel (nuclear stain); (C) emission from the green channel (NO probe); (D) emission from the red channel (mitochondrial stain); and (E) overlay of the green and red channels. Scale bars, 25 μm.
