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Schematic of synthesis of AuNP–BBN and 198AuNP–BBN conjugates (Upper) and amino acid sequence in thioctic acid–bombesin peptide (Lower). The available reactive sites on AuNPs allow incorporation of varying amounts of BBN peptides and provide a practical means of developing a library of AuNP–BBN analogs with one-, two-, and three-peptide equivalents per nanoparticle, resulting in AuNP–BBN-1, AuNP–BBN-2, and AuNP–BBN-3 for low/high capacity binding with GRP receptors.
