Fig. 4.
Interaction of BRCA2[BRC1-8] with the DNA-binding domain of p53. (A) Nickel pull-down experiment of his6 BRCA2[BRC1-8] with different domains of p53. Immunoblots were developed by using ab2957 (Abcam, specific for 1324–1347 of human BRCA2). (B) Overlay of 1H15N HSQC of labeled p53 DNA-binding domain (94–312) in the presence (Blue) and absence (Red) of BRCA2[BRC1-8]. (C) Chemical shift mapping of the residues on the p53 DNA-binding domain (PDB ID code 2AC0) that are perturbed upon BRCA2[BRC1-8] binding. p53 DBD is shown in green and DNA in black. Residues that showed chemical shift changes and those that disappeared are shown in red and blue, respectively. Selected residues that showed significant chemical shift perturbations are highlighted in magenta.