Fig. 5.

Transcriptional and apoptosis assays. (A) H1299 cells with inducible p53 expression were transfected with BRCA2, BRCA2_CTD, BRCA2 ΔCTD, or pcDNA (+)(empty) vectors as indicated. Expression of p53 was induced by placing the cells in medium containing no tetracycline. Bars 1–4 represent experiments where p53 was not induced (^∗P < 0.05). (B) Caspase-3 activity assay monitoring the apoptosis in H1299 cells transfected with BRCA2, BRCA2_CTD, BRCA2 ΔCTD, and pcDNA (+)vectors as indicated in A (^∗P < 0.05). (C) Expression levels of p53 target genes, p21 and Bax, when transfected with empty vector, BRCA2_CTD, BRCA2, and BRCA2 ΔCTD. (D) Western blot showing the overexpression of BRCA2 when tranfected in H1299 cells. p53 levels remained unaffected by BRCA2 overexpression as indicated by Western blot. The numbers represent the cell extracts from the table in A.