Table 1.

Dissociation constants of BRCA2 OB domains and ssDNA or p53 TAD

BRCA2 domains	ssDNA (nM)	p53N (μM)	p53 TAD2WT (μM)	p53 TAD2Mut1 (μM)	p53 TAD2Mut2 (μM)
OB1	10,000 ± 1,000	n.q.*	n.q.*	n.q.*	n.q.*
OB2	150 ± 7	5.2 ± 0.3	6.0 ± 0.5	50 ± 4	n.q.*
OB3	110 ± 6	2.5 ± 0.2	3.2 ± 0.4	43 ± 3	n.q.*
OB2 + OB3	40 ± 5	3.0 ± 0.4	4.0 ± 0.3	39 ± 5	120 ± 10

ssDNA (dT)₁₅, p53 TAD (1–93), p53TAD2 WT (35–57), p53TAD2 Mut1 (35–57): L43A/L45A/I50A/W53A/F54A and p53TAD2 Mut2 (35–57): D41K/D42K/D48K/D49K/E51K were used. Experiments were carried out in a buffer containing 25 mM Hepes, 150 mM NaCl, 3 mM DTT, pH 7.4 at 20 °C. The dissociation constant was obtained by fitting to a cooperative binding model and gave a Hill constant of ∼2.2.

^*n.q.: not quantified (> 250 μM).