Fig. 2.

PPARγ activation is required for expression of mDefB10 in the colon. (A) Immunohistochemical localization of mDefB10 in Pparγ^+/+ (Left) and Pparγ^−/− (Right) mice. (Scale bar: 10 μm.) (B) To avoid estrus variation, the proximal colon of 8-week-old Pparγ mutant males and their WT littermates (n = 4) were dissected out, flushed with cold PBS, and processed for expression analysis. (C) Potential PPARγ-binding sites within the mDefB10 promoter. (D) Relative gene expression in 8-week-old WT C57BL6/J female mice treated orally or rectally with rosiglitazone at a single or double dose of 10 mg/kg for 14 days compared with untreated mice (n = 4). Values represent the mean of normalized data ± SEM, as measured by real-time qPCR. P values were determined by the Mann-Whitney test. NS, not significant.