Fig. 4.

Reduced expression of DEFB1 is specifically linked to colonic involvement in CD. (A) Relative gene expression in macroscopically noninflamed colonic biopsy specimens from ileal (n = 21), colonic (n = 16), and ileocolonic (n = 21) CD patients compared with UC patients (n = 8) and controls (n = 17) who underwent colonoscopy. Values represent the mean of normalized data ± SEM, as measured by real-time qPCR. NS, not significant. P values were determined by the nonparametric Kruskall-Wallis and Mann-Whitney tests. (B) Correlation between DEFB1 and IL-8 transcript levels in CD. P values were determined by the nonparametric Spearman test. (C) Immunohistochemical localization of DEFB1 in control (Left) and Crohn's colitis patients (Right). (Scale bar: 10 μm.)