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. 2010 Jul;58(7):595–609. doi: 10.1369/jhc.2010.955294

Figure 1.

Figure 1

Nuclear receptor coactivator 4 (NcoA4) mRNA expression during mouse development and in different adult mouse tissues. Total RNA was extracted from homogenized embryonic day 9.5 (E9.5) to E18.5 whole mouse embryos (A) and from various adult mouse tissues (B), and RT-PCR was performed. Two PCR products migrating at 1.9 and 1.2 kb were detected. Automated nucleotide sequencing confirmed that the higher band corresponds to the published full-length mouse NcoA4 cDNA consisting of 1923 nucleotides (mNcoA4), whereas the lower band lacks 588 nucleotides (missing nt 293–768 and nt 787–898) (C). The NcoA4 protein contains the FXXLF signature motif at aa 334–338 (C). Alignment of these sequences with mouse chromosome 14 using Ensembl Mouse Genome Browser software indicated that murine NcoA4 consists of 10 exons, whereas the 1.2-kb mRNA excludes exons 4, 5, 6, and 7, and portions of exons 3 and 8 (D).