Figure 2.

NcoA4 protein expression during mouse development and in different adult mouse tissues. Total protein was extracted from homogenized E9.5–E17.5 whole mouse embryos and from various adult mouse tissues, and Western blot analysis for NcoA4 expression was performed. A 70-kDa immunoreactive band was detected at the later stages (E15.5–E17.5) of embryonic development (A). A 70-kDa immunoreactive band was also detected in all the adult tissues examined (B). NcoA4 protein levels were normalized using actin protein as a loading control. Representative blots are shown (A,B). The bar graphs summarize the results of these experiments expressed as the ratio of the 70-kDa intensity relative to actin.