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Analysis of the transformants after induction with galactose. (a) Electrophoresis on a 12 % SDS-polyacrylamide gel stained with Ponceau red. (b) Western immunoblots performed with anti-V5 antibody. Lanes: 1, total proteins from yeast cells expressing CYP619C2; 2, from yeast cells expressing CYP619C3; 3, from CYP619C2 control; 4, from CYP619C3 control. For both controls, each gene was inserted in the non-coding direction into the pYES vector with which the yeast cells were transformed.
