Table 1.

Primers used in this study

Primer*	Sequence (5′–3′)	Annealing temperature
P450Iext5′1	GGGTAGCTCAGGTGGCCGATATCCTGAGCGCA	60 °C
P450Iext5′2	TCAGCAGCACCCCGAGGATGTACGATGGCGG	60 °C
P450IIext5′1	CCAGTCGGCGATCCGTGACGACGGCC	60 °C
P450IIext5′2	GCCATTCCCAACTTTGAGCATGTAGAGGCCGCCG	60 °C
P450Iext3′1	CGTCTGGGGCATGCACCACGACCCCGACC	60 °C
P450Iext3′2	CGCTGGTCCGAACCGGAGCACTTCCAGCCC	60 °C
P450IIext3′1	GTGGGACGGCGCATCTGCCCGGGG	60 °C
P450IIext3′2	GGCGTTCGAGTTCCAGCGCGGGGAGG	60 °C
P450IexpF	AAGATGGATTTCACACAAGTACCGCCATCG	55 °C
P450IexpR	ATCAAACCGGCTAAACACCCCCTGG	55 °C
P450IIexpF	ACCATGGAACCAATGCTGCTCCTGATATTG	55 °C
P450IIexpR	GCCCTCCGTAAACTGCGAAAACACC	55 °C
CYP619C2F3	ACCTGAGCTACCCAGCGGA	60 °C
CYP619C2R3	GCCCATTTGGTGAACTGAAAA	60 °C
CYP619C3F2	TCAGTAACAGTATCACCAACTCAATCGTA	60 °C
CYP619C3R2	GGCGCCGGTTTCCATAAT	60 °C
TubACF3	GCCCTACAACGCCACTCTCTC	60 °C
TubACR3	GCATGCAGATATCGTACAGAGCC	60 °C

*P450I (CYP619C2) and P450II (CYP619C3) are equivalent, respectively, to sequences submitted under GenBank accession numbers XM_001273090 and XM_001273089.