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. 2009 Dec;155(Pt 12):3957–3970. doi: 10.1099/mic.0.032284-0

Fig. 2.

Fig. 2.

Macroscopic and microscopic characteristics of HI. (a) Growth of a heterokaryon between XK81 and FGSC 456 (temperature-sensitive incompatible, TS inc, row I) compared to growth of a heterokaryon between XK81 and Xa-3 (compatible; row C) grown at permissive temperature for HI (20 °C). Both heterokaryons were grown at 34 °C for 16 h (0 min) and then transferred to 20 °C, and photographs of heterokaryons growing on Petri dishes were taken at the time points indicated following shift to 20 °C. The arrow shows conidiation observed in the compatible heterokaryon after 8 h, while the TS inc heterokaryon shows no growth or conidiation during the same time period. (b) DIC micrographs of hyphae from the TS inc (row I) and compatible (row C) heterokaryons stained with the vital dye methylene blue and photographed prior to transfer (0 min) and at the indicated time points after transfer to 20 °C.