Table 1.
Strains and plasmid constructs used in the study
| Strain or plasmid | Description | Reference/source |
|---|---|---|
| Salmonella typhimurium | ||
| ST14028s | Wild-type and parental strain | Lu et al. (1999) |
| T-InvJ | invJ : : 1xFLAG | This study |
| T-PrgJ | prgJ : : 1xFLAG | This study |
| T-SipC | sipC : : 1xFLAG | This study |
| T-SipD | sipD : : 1xFLAG | This study |
| T-SopA | sopA : : 1xFLAG | This study |
| T-SopB | sopB : : 1xFLAG | This study |
| Escherichia coli | ||
| DH5α | F−φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17( ) phoA supE44 λ−thi-1 gyrA96 relA1
|
Invitrogen |
| Plasmids | ||
| pUC-H1PF1 | Apr Kanr, template plasmid for 1xFLAG epitope tag | Su et al. (2009) |
| pKD46 | Apr, containing the Red recombinase of λ phage | Datsenko & Wanner (2000) |
| pCP20 | Containing the expression cassette of flippase, which can remove the kanamycin-resistance cassette from the mutant strains | Datsenko & Wanner (2000) |