Table 2.

Primers used to construct the tagged strains

ORFs	Upstream primer	Downstream primer
invJ	5′-GCTGGCACCTGACGCGAGACGATC AACAAAATCCGCAGCAGCAACAGCA CAGACAGCAATCTGGCGAGGAGGAT GACGCCGATTACAAGGATGACGACGA-3′	5′-CCGGCCATGGCGCTGGCATTCTG TCGCGGTTTGCGCCAATAGCCATT CGCGACGATCAATCTGTCTCACAC GCAATGACACATATGAATATCCTC CTTAGTT-3′
prgJ	5′-CAGATTATAACCTGTATGTTTCTA TGGTCAGTACCCTTACTCGTAAAGG AGTCGGGGCTGTTGAAACGCTATTA CGCTCAGATTACAAGGATGACGACG A-3′	5′-GTCCAGTCCTTTTAAAAGATCCT TATCCTTACAGCCGGCAAGGGTCA TTACCAGCAGAAAAGTATATAGAT ATCGACGAACATATGAATATCCTCC TTAGTT-3′
sipC	5′-AGGAAATGCTGAAAACAATGGAG AGCATTAACCAGTCGAAAGCATCC GCACTCGCTGCTATCGCAGGCAATA TTCGCGCTGATTACAAGGATGACGA CGA-3′	5′-TATTAAGCATAATATCCCCAGTTC GCCATCAGGAGCGCGATTAAATCA CACCCATGATGGCGTATAGATGACC TTTCAGACATATGAATATCCTCCTTA GTT-3′
sipD	5′-CATTGTACGACAACCTGGTAAAA GTGCTGAGCAGTACGATAAGTAGC AGCCTGGAAACCGCCAAAAGCTTC CTGCAAGGAGATTACAAGGATGAC GACGA-3′	5′-TTGATCTCGGTCTGCATACCTGGC ATTATGACGGGGGGCTGAGTCCTTA CACTTGTAACCATTATTAATATCCTC TTCTGCATATGAATATCCTCCTTAGTT-3′
sopA	5′-TTGTGGCGGATAGTATGCAACGT CATGCCAGAAAATATTTCCCGAGT GTTCTGTCATCCATCCTGCCACTGG CCTGGGCGGATTACAAGGATGACG ACGA-3′	5′-AAAATGCATGGACTAAAACGGGT CTATGTACAGAGGGACACAACGCTG TGTCCCTTAATTCCATGCGGGTTGAG GCTGGACATATGAATATCCTCCTTAG TT-3′
sopB	5′-TCAATCTTTCCTATCAAAAACGA GTTGGGGATGAAAATATTTGGCAG TCAGTAAAAGGCATTTCTTCATTA ATCACATCTGATTACAAGGATGAC GACGA-3′	5′-GTGGCTCATCTTCTGGCGCATCCA GGCCTAACGCGTCATATAAACGATT TAATAGACTTTCCATATAGTTACCTC AAGACCATATGAATATCCTCCTTAGT T-3′