Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source/reference |
|---|---|---|
| E. coli O157 : H7 | ||
| TW08264 | WT strain Sakai | Michino et al. (1999) |
| EcJR-8 | SakairpoN : : kan | This study |
| EcJR-5 | SakairpoN : : kan pCR2.1(rpoN+) | This study |
| EcJR-6 | SakaiΔgadE rpoN : : kan | This study |
| EcJR-9 | SakaiΔrpoS rpoN : : kan | This study |
| TW04863 | WT strain 93-111 | STEC Center, Mich. State Univ. |
| EcJR-10 | 93-111rpoN : : kan | This study |
| TW14359 | WT | STEC Center, Mich. State Univ. |
| EcJR-11 | TW14359rpoN : : kan | This study |
| TW15901 | Sakai pKM208 | Kailasan Vanaja et al. (2009) |
| TW15902 | SakaiΔgadE | Kailasan Vanaja et al. (2009) |
| Plasmids | ||
| pCR2.1 | TOPO cloning vector | Invitrogen |
| pCR2.1(rpoN+) | pCR2.1 containing rpoN | This study |
| pKM208 | Red-recombinase expression vector | Datsenko & Wanner (2000) |
| pKD4 | Template plasmid for Kan cassette | Datsenko & Wanner (2000) |
| pCP20 | Flp recombinase expression vector | Datsenko & Wanner (2000) |