Table 2.
Differentially expressed genes in SakairpoN : : kan compared to Sakai
| ECs no.* | Gene | Function | Expression ratio SakairpoN : : kan/Sakai† | |
|---|---|---|---|---|
| Expo | Stat | |||
| Hypothetical and miscellaneous | ||||
| 0069 | yabI | Membrane-associated protein | 0.20 | – |
| 0781 | ybgS | Homeobox protein | 7.73 | – |
| 0982 | ycaC | Predicted hydrolase | 9.24 | – |
| 1152 | yccJ | Unknown | 3.24 | – |
| 1683 | ycgB | Putative sporulation protein | 5.59 | – |
| 1695 | yihV | Unknown | – | 0.05 |
| 1768‡ | Sp9-encoded protein | 4.21 | – | |
| 2316 | tus | DNA-replication protein | 3.63 | – |
| 2430 | ydiZ | Unknown | 3.88 | – |
| 2546 | yebV | Unknown | 20.0 | – |
| 2547 | yebW | Unknown | 5.85 | – |
| 2662 | fliC | Flagellin | 0.25 | – |
| 2692 | yodD | Unknown | 6.94 | – |
| 3154 | elaB | Unknown | 12.1 | – |
| 3413‡ | Unknown | 5.32 | – | |
| 4037 | yhbQ | Predicted endonucleases | 4.44 | – |
| 4291‡ | Unknown | 8.59 | – | |
| 4323 | yhhT | Inner-membrane protein | 0.07 | 0.30 |
| 4363 | yhiM | Unknown | 6.48 | – |
| 4699 | yifE | Unknown | 0.12 | – |
| 4737 | yzcX | Unknown | 5.81 | – |
| 4745 | yigE | Unknown | 2.65 | – |
| 4801‡ | Unknown | 3.17 | – | |
| Stress resistance | ||||
| 1829 | yciE | Unknown | 6.00 | – |
| 1830 | yciF | Structural protein | 13.5 | – |
| 1831 | yciG | Unknown | 9.39 | – |
| 1881§ | pspA | Phage-shock protein A | 0.28 | 0.04 |
| 1882§ | pspB | Phage-shock protein B | – | 0.14 |
| 1883§ | pspC | Phage-shock protein C | – | 0.34 |
| 1884§ | pspD | Phage-shock protein D | – | 0.36 |
| 1885§ | pspE | Phage-shock protein E | – | 0.49 |
| 2097 | gadC | Acid-sensitivity protein (XasA) | 5.28 | – |
| 2098 | gadB | Glutamate decarboxylase isozyme | 4.07 | – |
| 2604 | otsA | Trehalose-6-phosphate synthase | 4.46 | – |
| 2605 | otsB | Trehalose-6-phosphate phosphatase | 2.82 | – |
| 3186 | yfcG | Glutathione S-transferase | 3.23 | – |
| 3533 | ygaM | Unknown | 6.62 | – |
| 4377 | slp | Starvation lipoprotein | 3.29 | – |
| 4392 | gadE | Acid-responsive regulator | 3.83 | – |
| 4396 | gadX | Glutamate decarboxylase activator | 3.31 | – |
| 4397 | gadA | Glutamate decarboxylase isozyme | 3.49 | – |
| 5586 | ecnB | Bacteriolytic enterocidin B | 7.47 | – |
| Transport and metabolism | ||||
| 0504§ | glnK | Nitrogen regulatory protein P-II 2 | 0.01 | 0.02 |
| 0505§ | amtB | High-affinity ammonium transporter | 0.08 | 0.12 |
| 0538 | ybaS | Glutaminase | 5.03 | – |
| 0693§ | gltJ | Glutamate transporter, permease | 0.19 | – |
| 0694§ | gltI | Glutamate transporter, periplasmic | 0.08 | 0.07 |
| 0887§ | glnQ | ATP-binding protein for Gln transporter | 0.26 | – |
| 0888§ | glnP | Permease protein of Gln transporter | – | 0.30 |
| 0889§ | glnH | Periplasmic protein for Gln transporter | 0.07 | 0.09 |
| 1254§ | rutE | Nitroreductase | 0.19 | – |
| 1255§ | rutD | Putative acetyltransferase | 0.24 | – |
| 1711 | ychM | Sulfate permease | 5.73 | – |
| 1722 | chaB | Cation transport regulator | 7.70 | – |
| 1879 | puuE | GABA-aminotransferase | 4.06 | – |
| 1921 | abgB | p-Aminobenzoyl-glutamate hydrolase | – | 0.22 |
| 1922 | abgA | Putative aminohydrolase | – | 0.06 |
| 2082 | adhP | Ethanol dehydrogenase | 7.50 | – |
| 2091§ | ddpA | Putative d-Ala-d-Ala transport protein | 0.09 | – |
| 2092§ | ddpX | Putative d-Ala-d-Ala dipeptidase | 0.09 | – |
| 2103 | ydeN | Predicted sulfatase | 0.12 | – |
| 2451§ | astB | Succinylarginine dihydrolase | 0.25 | – |
| 2452§ | astD | Succinylglutamic semialdehyde dehydrolase | 0.15 | – |
| 2453§ | astA | Arginine succinyltransferase | 0.21 | – |
| 2454§ | astC | 0.20 | – | |
| 2650 | pgsA | Phosphatidylglycerophosphate synthetase | 2.93 | – |
| 2784§ | nac | Nitrogen assimilation control protein | 0.03 | 0.08 |
| 2900 | Fructose bisphosphate aldolase | 7.65 | – | |
| 3058 | yeiC | Predicted kinase | 0.27 | |
| 3192§ | hisQ | Permease for histidine transport | 0.28 | – |
| 3193§ | hisJ | Periplasmic protein for histidine transport | 0.15 | – |
| 3327 | tktB | Transketolase | 8.50 | – |
| 3425 | tadA | tRNA-specific adenosine deaminase | 5.13 | – |
| 3689 | ygdQ | TerC-like transport protein | 0.06 | 0.09 |
| 4141§ | yhdW | Periplasmic-binding protein | 0.38 | – |
| 4142§ | yhdX | Transport system permease protein | 0.46 | – |
| 4144§ | yhdZ | ATP-binding protein | 0.29 | – |
| 4448 | xylA | Xylose isomerase | 0.17 | – |
| 4490 | yibO | Phosphoglyceromutase | 7.18 | – |
| 4492 | yibQ | 2.48 | – | |
| 4734 | hemD | Uroporphyrinogen-III synthetase | 2.54 | – |
| 4735 | hemC | Porphobilinogen deaminase | 2.07 | – |
| 4790§ | glnG | Glutamine utilization response regulator | 0.15 | – |
| 4791§ | glnL | Glutamine utilization sensor kinase | 0.08 | 0.34 |
| 4792§ | glnA | Glutamine synthetase | 0.08 | 0.07 |
| Transcription and translation | ||||
| 1902 | tyrR | Transcriptional dual regulator | 6.36 | – |
| 2032 | rimL | Ribosomal-serine N-acetyltransferase | 4.59 | – |
| 2084 | rpsV | 30S ribosomal protein S22 | 6.34 | – |
| 2783 | cbl | CysB-like regulator of cys operon | 0.09 | 0.18 |
| 3136 | yfaX | Putative regulator | 0.12 | – |
| 3403 | hcaR | Transcriptional activator of hca cluster | 2.75 | – |
| 4440 | yiaG | Transcriptional regulator | 7.66 | – |
| 4484 | yibK | rRNA methylase | 4.51 | – |
| 4867 | metJ | Methionine transcriptional repressor | 2.40 | – |
| Pathogenesis | ||||
| 0780 | zitB | Zinc transport/adhesin | 0.36 | – |
| 3704‡ | yqeI | ETT2 sensory transducer | 3.91 | – |
| 4551‡ | orf29 | LEE4 type III secretion protein | 0.33 | – |
| 4554‡ | espB | LEE4 translocon protein | 0.29 | – |
| 4560‡ | cesT | LEE5 type III secretion chaperone | 0.17 | – |
| 4561‡ | tir | LEE5 translocated intimin receptor | 0.26 | – |
| 4563‡ | cesF | LEE3 type III secretion chaperone | 0.32 | – |
| 4491 | envC | Murien hydrolase | 4.31 | – |
| 4571‡ | espZ | LEE2 type III secretion protein | 0.24 | – |
*Locus tag (ECs no.) for E. coli O157 : H7 strain Sakai (GenBank no. BA000007).
†Expression ratio for exponential (Expo) and early stationary (Stat)
phase cultures of SakairpoN : : kan
and Sakai determined as 
. –,
No significant differential expression between SakairpoN : : kan and Sakai.
‡Gene is encoded within an E. coli O157 : H7-specific sequence.
§Gene previously shown to be regulated by RpoN.