Table 3.

Validation of microarray genes by qRT-PCR

Gene	Expression ratio (SakairpoN : : kan/Sakai)* Expo. (OD600=0.5)
	qRT-PCR	MA†
cesT	0.16±0.03‡	0.17
espA	0.29±0.06	–
espB	0.14±0.03	0.29
espZ	0.20±0.05	0.24
ler	–	–
tir	0.15±0.04	0.26
gadC	33.3±13.0	5.28
gadE	4.00±1.00	3.83

*Normalized gene expression ratios for SakairpoN : : kan/Sakai during exponential (Expo.) phase growth in DMEM-MOPS. Gene expression ratio was calculated as . –, Not significantly altered in expression.

†MA is gene expression ratio for microarray data from Supplementary Table S2.

‡95 % confidence interval on the mean (n=3).