Fig. 3.

Binding of RTA to the ORF57 promoter RREs in vivo. (a) A transient ChIP assay was carried out in 293T cells transfected with the reporter plasmids p57-3RRE and p57-3RRED3 as indicated, together with Flag-tagged full-length RTA expression plasmid. RTA–DNA complexes were specifically precipitated with or without anti-Flag antibody. The precipitated DNA was recovered by PCR with specific primers located in the backbone of the reporter vector. (b) Taqman real-time PCR was performed to quantify the immunoprecipitated promoter DNA from transfected 293T and TRExBJAB-RTA cells.