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. 2010 May;156(Pt 5):1424–1438. doi: 10.1099/mic.0.036640-0

Fig. 2.

Fig. 2.

Effect of heat treatment of HmsH and HmsF on migration in polyacrylamide gels. Samples were suspended in sample buffer containing 2 % SDS, heated at 100 °C for 10 min or unheated (RT; ∼20 °C), then resolved by 8 % SDS-PAGE. (a) Western blot probed with HmsH antisera. Arrows identify HmsH in boiled and unboiled samples. (b) Western blot probed with HmsF antisera. The upper arrow indicates the unprocessed form of HmsF, while the lower one indicates the processed form. Strains (see also Table 1): Δpgm, KIM6; hms+, KIM6+; ΔhmsH, KIM6-2115; ΔhmsF, KIM6-2116.