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CR binding by HmsH-D263A and HmsH-R479A variants is temperature-dependent. Quantitative CR binding is shown for Y. pestis KIM6-2115 (in-frame ΔhmsH; HmsH−), KIM6-2115(pNPM22) (HmsH++) and the two variants after 3 h incubation at 20 °C (a) or 30 °C (b) with CR-containing HIB medium. Data shown are the mean of two or more independent experiments with duplicate samples from each trial. Error bars, sd.
