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Western blot of formaldehyde-cross-linked Y. pestis strains probed with HmsH antisera. Strains: Y. pestis KIM6+ (Hms+), KIM6-2115 (ΔhmsH), KIM6-2115(pNPM22) (hmsH++), KIM6-2116(pBADhmsF) (hmsF++) and KIM6-2116 (ΔhmsF). Black arrows identify the two HmsH–HmsF cross-linked complexes, while the grey arrow shows the HmsH monomer. Molecular masses of protein standards are shown within the range of 250–98 kDa.
