Fig. 2.

Mutation of the 4.5S RNA alters the GAS secretome. Secreted proteins from GAS strains MGAS2221 (+vector), 2221Δ4.5S (+vector) and 2221Δ4.5S (+p4.5S) were ethanol precipitated and subjected to Western blot analyses. Exponential-phase samples were recovered at an OD₆₀₀ of 0.5, while stationary-phase samples were recovered at an OD₆₀₀ of 1.7. All Western blots gave signals at the expected locations given the molecular masses of the target proteins [Sic (35 kDa), SLO (64 kDa), Spd3 (30 kDa) and SpeB (43 kDa)].