Fig. 3.

The protein components of the pheromone response network constructed by the PCST approach. Note that the canonical pheromone response pathway (enclosed by dashed lines) is but a small component of the broad cellular changes revealed by applying the algorithm to the mass spectrometry and expression data. For clarity the differentially transcribed genes included in the network are not presented. Functional groups based on GO annotation are outlined with red boxes. PKC, protein kinase C; TF with phos. site, transcription factor with at least one differentially phosphorylated sites; TF with no phos. site, transcription factor with no differentially phosphorylated sites; non-TF protein with phos. site, a protein that is not a transcription factor and with at least one differentially phosphorylated sites; non-TF with no phos. site, a protein that is not a transcription factor and with no differentially phosphorylated sites.