. 2009 Dec 10;8(2):209–226. doi: 10.2203/dose-response.09-016.Nowosielska

TABLE 4.

Inhibition of the cytotoxic activity [expressed in % lysis] of the NK cell-enriched spleno-cytes (NK cells – inhibited by CMA and anti-FasL Ab) and the IFN-γ- and LPS-stimulated peritoneal macrophages (Mϕ – inhibited by CGN and AG) on the 2nd and 3rd days after single WBI (Fig. 1) with X-rays, respectively.

	Cytotoxic activity of NK cells [% lysis]				Cytotoxic activity of Mϕ [% lysis]
Groups	CM	CMA	anti-FasL Ab	CMA + anti-FasL Ab	PBS	CGN	AG	CGN + AG
Control	7.2	2.4^*	4.5^*	0.6^*	29.4	14.6 ^a	4.2 ^a	3.8 ^a
0.1 Gy	11.1^{^}	3.5^*^{^}	6.8^*^{^}	0.4^*	40.4 ^b	16.3 ^a	8.3 ^a ^b	7.9 ^a ^b
0.2 Gy	11.4^{^}	3.5^*^{^}	7.2^*^{^}	1.1^*^{^}	42.1 ^b	17.5 ^a	9.5 ^a ^b	8.7 ^a ^b

Control – sham-exposed mice; 0.1 Gy – mice exposed to a single WBI with 0.1 Gy X-rays; 0.2 Gy –mice exposed to a single WBI with 0.2 Gy X-rays.

CM – NK cells incubated in culture medium without blockers; CMA – NK cells incubated with CMA; anti-FasL Ab – NK cells incubated with anti-FasL antibody; CMA + anti-FasL Ab – NK cells incubated with CMA and anti-FasL antibody.

^{^}

indicates statistically significant (p<0.05) difference between NK cells collected from irradiated mice and the respective NK cells obtained from non-irradiated mice.

indicates statistically significant (p<0.05) difference within each sham-irradiated or irradiated group between NK cells incubated with CMA and/or anti-FasL antibody and NK cells incubated without blockers (CM group).

PBS – Mϕ obtained from mice pretreated with PBS; CGN – Mϕ obtained from mice pretreated with CGN; AG – Mϕ obtained from mice pretreated with PBS and incubated in vitro in the presence of AG; CGN + AG – Mϕ obtained from mice pretreated with CGN and incubated in vitro in the presence of AG.

indicates statistically significant (p<0.05) difference within sham-irradiated or irradiated groups between Mϕ incubated with AG and/or obtained from mice pretreated with CGN and Mϕ collected from mice pretreated with PBS and incubated without AG.

indicates statistically significant (p<0.05) difference between Mϕ collected from irradiated mice and the respective Mϕ obtained from non-irradiated mice.