Fig. 3.

Conditional deletion of HDAC1 or -2 does not inhibit the growth or differentiation capacity of ES cells. (A) Colony formation assay: mean values and SDs of cells of the indicated genotype plated at low density and cultured for 8 days, before counting. Mean values (n = 3) ± SEM are plotted. (B) The population doubling times of the indicated cell types were calculated by counting and then replating cells every 2 days for an 18-day period. (C) Differentiation potential. ES cells of the type indicated were plated at low density in the presence (+) or absence (−) of LIF and cultured for 6 days before staining for the presence of alkaline phosphatase. Colonies were scored as undifferentiated (purple), differentiated (white), or mixed. Data represent three independent experiments using two individual clones of each genotype.