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. 2010 Apr 12;107(18):8111–8116. doi: 10.1073/pnas.1003056107

Fig. 1.

Fig. 1.

Characterization of the Aedes aegypti hybrid transgenic strain expressing both CecA and DefA. (A) Northern blot analysis of the Vg-CecA C32, Vg-DefA X1, and Vg-CecA/Vg-DefA CxD hybrid mosquitoes. The transgenic and control wild-type (wt) mosquitoes were analyzed 24 h PBM. Total RNA was hybridized with CecA, DefA, or actin cDNA probes wt parental strain. (B) MALDI-TOF mass spectrometry analysis of the hemolymph of the hybrid CxD (Upper Panel) and parental wt (Lower Panel) mosquitoes. Peptide sequences below MALDI-TOF image represented processed mature 34-mer and 35-mer Cec A and 40-mer DefA peptides. (C) Survival rate of transgenic and control wt mosquitoes after infection with P. aeruginosa. Means and standard deviations of results reflect data from two independent experiments. P value between the CxD hybrid with other groups was < 0.001. (D) Bacterial proliferation test for P. aeruginosa in adult transgenic and wt mosquitoes. Three mosquitoes per sample were collected and used for colony forming unit determination. Analysis of wt, the transgenic C32, X1, and CxD hybrid female mosquitoes was preformed. Means and standard deviations of results reflect data from two independent experiments. Asterisk (*) represents P < 0.05 (t test), while CxD was compared to other groups.