Fig. 3.

RNA interference depletion of CecA to test the functional effect of CecA overexpression on P. gallinaceum infection. Wt and transgenic Vg-CecA female mosquitoes (C32 strain) were injected with CecA or control luciferase dsRNA. One week later mosquitoes from each treated group were blood fed on the same infected chick. Mosquito midguts were dissected 7 days postinfection and scored for oocyst numbers: Vg-CecA/iLuc, Vg-CecA transgenic mosquitoes treated with luciferase dsRNA (control); Vg-CecA/iCecA, Vg-CecA transgenic mosquitoes treated with CecA dsRNA; wt/iLuc, wt mosquitoes treated with luciferase dsRNA (control for infection). Data represents the result of 3 independent experiments for each transgenic and control strains.