Table 2.
Blocking of Plasmodium transmission in transgenic mosquitoes co-overexpressed DefA and CecA
| Wild-type | Vg-CecA | Vg-DefA | Vg-CecA/DefA | ||
| Exp 3 | *Sporozoite # per a SG | 12210 (8410–17430) | 350 (0–560) | 660 (40–1240) | 0 (0–0) |
| †Parasitemia (%)/chicks # | 15% (5–20%)/9 | 1% (0–4%)/9 | 3% (0–9%)/8 | 0% (0–0%)/11 | |
| ‡Oocyst # per a midgut | 42 (11–79) | 1 (0–6) | 2 (0–11) | 0 (0–0) | |
| Exp 4 | *Sporozoite # per a SG | 8950 (5840–15740) | 315 (0–610) | 573 (30–970) | 0 (0–0) |
| †Parasitemia (%)/chicks # | 12% (0–18%)/10 | 0.5% (0–2%)/9 | 2.1% (0–4%)/9 | 0% (0–0%)/10 | |
| ‡Oocyst # per a midgut | 29 (0–62) | 1 (0–4) | 1.5 (0–7) | 0 (0–0) | |
| Exp 5 | *Sporozoite # per a SG | 12950 (7820–17210) | 380 (0–770) | 1542 (10–11200) | 0 (0–0) |
| †Parasitemia (%)/chicks # | 14% (0–22%)/9 | 2% (0–3%)/9 | 3% (0–5%)/9 | 0% (0–0%)/10 | |
| ‡Oocyst # per a midgut | 34 (0–88) | 1 (0–5) | 2 (0–12) | 0 (0–0) |
*Plasmodium-infected mosquitoes from independent cohorts of EXP3-5 (Table 1) were fed on naive chickens on day 14 postinfection. This was done to reactivate the production of CecA and DefA in transgenic strains. The mean sporozoite number per a salivary gland. The range of observed values is indicated in parentheses.
†Mosquitoes from the same batches were then fed on naive chickens. Parasitemia of these chickens was established by examining red blood cells for the presence of Plasmodium infection. Parasitemia is expressed as the mean percentage of infected to total red blood cells examined. The range of observed value in an individual chick is indicated in parentheses.
‡Wt naive mosquitoes were blood fed on an individual chicken tested for parasitemia levels in each column of†. The oocyst number in there midguts was 7 days after blood feeding. The mean oocyst number per midgut and the range of observed values (in parentheses) are indicated.