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MLCK regulates ZO-1 exchange in vivo. (A) mRFP1-ZO-1 FRAP was assessed in wild-type (WT), long MLCK−/−, and CA-MLCK transgenic mice. PIK (250 μM) was added 15 min before analysis. (Scale bar, 3 μm.) (B) Mobile fraction of mRFP1-ZO-1, n = 5 per condition. § P < 0.001 vs. corresponding mucosa after PIK treatment.
