Fig. 1.

Novel synthetic small molecules dramatically increase hESC survival after single cell dissociation by enhancing cell-ECM mediated integrin activity. (A) Chemical structures of Thiazovivin/Tzv and Pyrintegrin/Ptn as indicated. (B) ALP staining of hESC colonies that had grown from dissociated single cells seeded in low density for four days and treated as indicated. Bar, 20 μm. (C) Ratio of ALP positive colonies vs. total initially seeded cells (n = 2). (D) Immunostaining of hESCs long-term maintained in chemically defined media containing Ptn or Tzv as indicated. Bar, 50 μm. (E) Representative phase contrast images of hESCs 12 h after seeding on the different matrices and treated with the indicated compounds. Bar, 20 μm. If not specified, all the above hESCs were grown in the chemically defined medium and feeder-free condition on Matrigel coated plates. All error bars indicate ± SEM.