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. 2010 Apr 20;107(18):8129–8134. doi: 10.1073/pnas.1002024107

Fig. 4.

Fig. 4.

Cell-cell interaction regulates cell-ECM interaction and contributes to survival and self-renewal of hESCs. (A) Western blot showing the active Rho level in hESCs before or after trypsin. (B) Rho-kinase assay showing the ROCK activity in hESCs before or after trypsin treatment (n = 3). (C) Western blot showing the active Rho level in hESCs 30 min after replating at the two different densities on the Matrigel-coated plates. (D) Rho-kinase assay showing the ROCK activity in hESCs 30 min after replating at the two different densities on Matrigel-coated plates (n = 3). (E) Western blot showing the active Rho level in hESCs 30 min after replating on BSA or different concentrations of E-cad-Fc chimera-coated plates. (F) Cell attachment on Matrigel-coated plates for different densities of hESCs (n = 2). LC, low-density culture (1.5 × 104 cells per well of 6-well plate). HC, higher-density culture (15 × 104 cells per well of 6-well plate). All graphs show mean ± SEM.