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. 2010 Jul 15;137(14):2279–2287. doi: 10.1242/dev.050427

Fig. 5.

Fig. 5.

Rat EG cells exhibit multilineage differentiation capacity in vitro. (A) Phase contrast image of day 4 embryoid body (EB). (B) RT-PCR analysis of undifferentiated EG cells and EBs after 4 and 8 days of differentiation. (C) Phase contrast and immunofluorescence images of an adherent EB, fixed and immunostained following 21 days of cardiac differentiation. Cardiomyocyte marker myosin colocalises with regions that exhibited spontaneous contraction prior to fixation. (D) Immunostaining for TuJ1 after 10 days of adherent monolayer neural differentiation on laminin. Scale bars: 100 μm in C; 50 μm in D.