Fig. 5.

drag-1 is localized to and functions at the cell membrane. (A) Schematics of various DRAG-1 deletion constructs (see Material and methods). (B-M) GFP localization (B,E,H,K; arrowheads point to the surface of the pharynx), the corresponding DIC images (C,F,I,L) and body size measurement (D,G,J,M) of animals containing the specific transgenes. DRAG-1::GFP (B-D), DelC::GFP (E-G; abbreviated as DelC), DelNC::GFP (H-J; abbreviated as DelNC) and LIN-12TM::GFP (K-M; abbreviated as LIN-12TM). In panels D, G, J and M: blue bars, drag-1(jj4) animals carrying the transgene; grey bars, drag-1(jj4) non-transgenic animals. The y-axis shows the relative body length, with the lengths of non-transgenic worms of each group being normalized to 1. Between 30 and 70 animals were measured for each genotype. Error bars represent 95% confidence intervals for the mean relative body length. The significance of difference between transgenic and the corresponding non-transgenic animals was statistically analyzed via Student's t-test. ***, P<0.0001. **, P<0.001. (N) Western blots probed with anti-GFP antibodies showing the localization of DRAG-1::GFP fusions via fractionation experiments. Asterisks refer to non-specific bands recognized by the anti-GFP antibodies. (O) Rescue of the mesodermal phenotype of jj4 mutants by various drag-1::gfp constructs. M-CC, M-derived coelomocytes.