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. 2010 Jul 15;137(14):2417–2426. doi: 10.1242/dev.047993

Fig. 4.

Fig. 4.

Slit-U rescues the slit mutant phenotype. (A) Schematic drawing of Slit. The arrow indicates the cleavage site. Slit-U or Slit-FL were expressed in HEK293T cells. Total lysates of the cells were harvested and subjected to polyacrylamide gel electrophoresis (PAGE). (B,C) Western blotting with antibodies to either the N- or the C-terminus of Slit indicates that Slit-U is not processed in 293T cells (B) or in embryos (C). (D) Quantification of segments in which FasII-positive neurons cross the midline. Error bars indicate s.d. (E-H) Stage 16 embryos were stained with a mAb to FasII (1D4). Anterior is up. (E) A wild-type embryo stained with mAb 1D4. (F) A slit mutant embryo. All axons collapse on the midline and there are no longer any distinct fascicles (asterisk). Expression of UASslit-FL (G) or UASslit-U (H) driven by slitGal4 in a slit embryo mostly rescues the aberrant midline crossing phenotype, but does not restore proper lateral positioning of the FasII-positive fascicles (arrow with asterisk).