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. 2010 Jul 15;137(14):2439–2449. doi: 10.1242/dev.049551

Fig. 2.

Fig. 2.

Integrin α5/αv endothelial conditional double-knockout (α5/αv-cdKO) embryos develop severe edema at ~E14.5 but have normal vasculature at earlier stages. (Aa-c) Most mice doubly deficient for α5 and αv integrins in endothelial and immune cells show edema (arrows) and hemorrhage at ~E14.5 (α5f/− αvf/− Tie versus α5f/+ αvf/+ Tie control). (B) Whole-mount vascular staining for (a,b) PECAM1 (HRP) or for (c-f′) β-galactosidase (X-gal) using the R26R-lacZ reporter strain crossed into the experimental crosses in control conditional doubly hemizygous and conditional double-knockout embryos (as labeled) at E10.5 and E11.5. Neither method reveals any obvious vascular defects in early embryos deficient for integrins. The boxed regions in e and f are shown at higher magnification in e′ and f′. Scale bars: 1 mm.