Fig. 3.
Immunoblotting for the protein expression of NADPH oxidase subunits in wild-type and A2AR knockout hearts. (A) A neutrophil membrane preparation was used as a positive control for the detection of Nox2. Cardiac troponin I was used as a loading control. (B) Protein bands were quantified densitometrically and normalised to the expression of cardiac troponin I in the same sample. The results were expressed as arbitrary units. *P<0.05 for A2AR knockout versus wild-type controls. n=12.