(A) Total cell counts, (B) Neutrophil percentages, and (C) IL-8 levels in BAL liquid obtained from non-CF and CF piglets within 6-12 h following birth. Each point represents a sample from a different animal. Horizontal line denotes median. No statistically significant differences were observed between non-CF and CF BAL liquid total cell counts, neutrophil percentages, or IL-8 levels. (D) Unsupervised hierarchical clustering of mRNA samples from trachea, bronchus, and distal lung samples obtained from newborn piglets (6-12 h old). Samples segregated into two clusters: airways (trachea and bronchus) and distal lung, but there was no clustering based on CFTR genotype. M and F refer to male and female pigs.